Journal: Molecular Vision

Article Title: Anti-allergic effects of mapracorat, a novel selective glucocorticoid receptor agonist, in human conjunctival fibroblasts and epithelial cells

doi:

Figure Lengend Snippet: Induction of allergy-related cytokines by interleukin-4/interleukin-13 and tumor necrosis factor-α in human conjunctival epithelial cells. Cells were treated with tumor necrosis factor-α (TNF-α), interleukin-4 (IL-4), and IL-13 alone or in the indicated combinations for 24 h. Cytokine/chemokine levels in the media were analyzed with Luminex. Data are presented as mean ± standard error of the mean (SEM), n=3. * versus control; † versus calculated combined effect of IL-4/IL-13 and TNF-α; p<0.05.

Article Snippet: Primary human conjunctival epithelial cells (HConEpiC) and primary human conjunctival fibroblasts (HConF) were received at passage 1 from ScienCell.

Techniques: Luminex, Control

Journal: Molecular Vision

Article Title: Anti-allergic effects of mapracorat, a novel selective glucocorticoid receptor agonist, in human conjunctival fibroblasts and epithelial cells

doi:

Figure Lengend Snippet: Induction of cytokines and ICAM-1 by IL-4 or IL-13 + TNF-α in HConEpiC and HConF.

Article Snippet: Primary human conjunctival epithelial cells (HConEpiC) and primary human conjunctival fibroblasts (HConF) were received at passage 1 from ScienCell.

Techniques:

Journal: Molecular Vision

Article Title: Anti-allergic effects of mapracorat, a novel selective glucocorticoid receptor agonist, in human conjunctival fibroblasts and epithelial cells

doi:

Figure Lengend Snippet: Mapracorat inhibits interleukin-4 plus tumor necrosis factor-α-induced cytokines and intercellular adhesion molecule 1 in human conjunctival epithelial cells. Cells were treated with interleukin-4 (IL-4) plus tumor necrosis factor-α (TNF-α) in the presence of mapracorat or dexamethasone for 24 h. Cytokine/chemokine levels in the media and intercellular adhesion molecule 1 (ICAM-1) in the cell lysates were analyzed with Luminex. Gray bar represents control; black bar represents IL-4 plus TNF-α; black circles plus line represent dexamethasone; white circles plus line represent mapracorat. Lines are the result of a reparameterized four-parameter logistic equation fit to the data. Data are presented as mean ± standard error of the mean (SEM), n=3. * versus IL-4 plus TNF-α; p<0.05.

Article Snippet: Primary human conjunctival epithelial cells (HConEpiC) and primary human conjunctival fibroblasts (HConF) were received at passage 1 from ScienCell.

Techniques: Luminex, Control

Journal: Molecular Vision

Article Title: Anti-allergic effects of mapracorat, a novel selective glucocorticoid receptor agonist, in human conjunctival fibroblasts and epithelial cells

doi:

Figure Lengend Snippet: Effect of glucocorticoid receptor silencing on mapracorat inhibition of interleukin-4/interleukin-13 plus tumor necrosis factor-α-induced cytokines and intercellular adhesion molecule 1 in human conjunctival epithelial cells and human conjunctival fibroblasts. After glucocorticoid receptor (GR) knockdown, cells were treated with interleukin-4 (IL-4) plus tumor necrosis factor-α (TNF-α) (human conjunctival epithelial cells [HConEpiC]) or IL-13 plus TNF-α (human conjunctival fibroblasts [HConF]) in the presence of mapracorat or dexamethasone. A : GR protein expression was determined with western blotting. B : Regulated on activation, normal T cell expressed and secreted (RANTES) levels and intercellular adhesion molecule 1 (ICAM-1) expression in HConEpiC were determined by Luminex. C : RANTES and eotaxin-1 levels in HConF were determined by Luminex. Left panels show data for control small interfering RNA (siRNA); right panels show data for GR siRNA. Data are presented as mean ± standard error of the mean (SEM), n=4. * versus IL-4/IL-13 plus TNF-α; p<0.05.

Article Snippet: Primary human conjunctival epithelial cells (HConEpiC) and primary human conjunctival fibroblasts (HConF) were received at passage 1 from ScienCell.

Techniques: Inhibition, Knockdown, Expressing, Western Blot, Activation Assay, Luminex, Control, Small Interfering RNA

Journal: Pharmaceutics

Article Title: Topical Insulin Eye Drops: Stability and Safety of Two Compounded Formulations for Treating Persistent Corneal Epithelial Defects

doi: 10.3390/pharmaceutics16050580

Figure Lengend Snippet: Serial slit-lamp images of a neurotrophic persistent epithelial defect treated with compounded insulin eye drops in isotonic saline solution (Inclor), Patient 9. ( A ) Baseline; ( B ) 4 days after initiating topical insulin treatment; ( C ) after 15 days’ treatment; and ( D ) after 22 days, when the epithelium healed.

Article Snippet: Likewise, immortalized human conjunctival epithelial cells (HConEpiCs; Innoprot, Bizkaia, Spain) were maintained under the same temperature, humidity, and CO 2 conditions, while the medium was changed every 48 h. HConEpiCs were cultured using the IM-Ocular Epithelial Cell Medium Kit (Innoprot, Bizkaia, Spain).

Techniques: Eye Drops, Saline

Journal: Molecular Vision

Article Title: Anti-allergic effects of mapracorat, a novel selective glucocorticoid receptor agonist, in human conjunctival fibroblasts and epithelial cells

doi:

Figure Lengend Snippet: Induction of cytokines and ICAM-1 by IL-4 or IL-13 + TNF-α in HConEpiC and HConF.

Article Snippet: Primary human conjunctival epithelial cells (HConEpiC) and primary human conjunctival fibroblasts (HConF) were received at passage 1 from ScienCell.

Techniques:

Journal: Molecular Vision

Article Title: Anti-allergic effects of mapracorat, a novel selective glucocorticoid receptor agonist, in human conjunctival fibroblasts and epithelial cells

doi:

Figure Lengend Snippet: Induction of allergy-related cytokines by interleukin-4/interleukin-13 and tumor necrosis factor-α in human conjunctival fibroblasts. Cells were treated with tumor necrosis factor-α (TNF-α), interleukin-4 (IL-4), and IL-13 alone or in the indicated combinations for 48 h. Cytokine/chemokine levels in the media were analyzed with Luminex. Data are presented as mean±standard error of the mean (SEM), n=3. * versus control; † versus calculated combined effect of IL-4/IL-13 and TNF-α; p<0.05.

Article Snippet: Primary human conjunctival epithelial cells (HConEpiC) and primary human conjunctival fibroblasts (HConF) were received at passage 1 from ScienCell.

Techniques: Luminex, Control

Journal: Molecular Vision

Article Title: Anti-allergic effects of mapracorat, a novel selective glucocorticoid receptor agonist, in human conjunctival fibroblasts and epithelial cells

doi:

Figure Lengend Snippet: Mapracorat inhibits interleukin-13 plus tumor necrosis factor-α-induced cytokines and intercellular adhesion molecule 1 in human conjunctival fibroblasts. Cells were treated with interleukin-13 (IL-13) plus tumor necrosis factor-α (TNF-α) in the presence of mapracorat or dexamethasone for 48 h. Cytokine and intercellular adhesion molecule 1 (ICAM-1) levels in the media were analyzed with Luminex. Gray bar represents control; black bar represents IL-13 plus TNF-α; black circles plus line represent dexamethasone; white circles plus line represent mapracorat. Lines are the result of a reparameterized four-parameter logistic equation fit to the data. Data are presented as mean ± standard error of the mean (SEM), n=3. * versus IL-13 plus TNF-α; p<0.05.

Article Snippet: Primary human conjunctival epithelial cells (HConEpiC) and primary human conjunctival fibroblasts (HConF) were received at passage 1 from ScienCell.

Techniques: Luminex, Control

Journal: Molecular Vision

Article Title: Anti-allergic effects of mapracorat, a novel selective glucocorticoid receptor agonist, in human conjunctival fibroblasts and epithelial cells

doi:

Figure Lengend Snippet: Effect of glucocorticoid receptor silencing on mapracorat inhibition of interleukin-4/interleukin-13 plus tumor necrosis factor-α-induced cytokines and intercellular adhesion molecule 1 in human conjunctival epithelial cells and human conjunctival fibroblasts. After glucocorticoid receptor (GR) knockdown, cells were treated with interleukin-4 (IL-4) plus tumor necrosis factor-α (TNF-α) (human conjunctival epithelial cells [HConEpiC]) or IL-13 plus TNF-α (human conjunctival fibroblasts [HConF]) in the presence of mapracorat or dexamethasone. A : GR protein expression was determined with western blotting. B : Regulated on activation, normal T cell expressed and secreted (RANTES) levels and intercellular adhesion molecule 1 (ICAM-1) expression in HConEpiC were determined by Luminex. C : RANTES and eotaxin-1 levels in HConF were determined by Luminex. Left panels show data for control small interfering RNA (siRNA); right panels show data for GR siRNA. Data are presented as mean ± standard error of the mean (SEM), n=4. * versus IL-4/IL-13 plus TNF-α; p<0.05.

Article Snippet: Primary human conjunctival epithelial cells (HConEpiC) and primary human conjunctival fibroblasts (HConF) were received at passage 1 from ScienCell.

Techniques: Inhibition, Knockdown, Expressing, Western Blot, Activation Assay, Luminex, Control, Small Interfering RNA